Uptake of taurocholate by isolated γ-glutamyltranspeptidase positive, putatively preneoplastic hepatocytes from 2-acetylaminofluorene treated rats

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Abstract
Uptake of the bile acid taurocholate was determined in liver cells isolated from male Wistar rats fed a standard diet or a diet containing 2-acetylaminofluorene (2-AAF). In addition, uptake was analysed in unaltered, γ-glutamyltranspeptidase (γ-GTase) negative, and in putatively preneoplastic, γ-GTase positive, hepatocytes separated from the total liver cell preparation isolated from the 2-AAF-treated animals. Total hepatocytes from the carcinogen-treated rats showed a - 50%decrease in the maximum rate of taurocholate transport compared to cells from untreated animals. Bile acid uptake in γ-GTase positive and negative liver cells revealed that V max was decreased by 44 ± 14% in the preneoplastic cell population. Since the K m value did not differ significantly it appears that the number of carrier molecules is reduced in the early preneoplastic hepatocytes. Our results show a partial loss of a liver-specific function in early preneoplastic γ-GTase positive hepatocytes.

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