The Amino Acid Requirement of the Laying Hen II. Classification of the Essential Amino Acids Required for Egg Production

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Abstract
The classification of amino acids according to their essentiality for the laying hen has been studied by use of a free amino acid diet. Arginine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine were found to be essential for egg production. With the exception of glutamic acid, omission of any one of the above amino acids resulted in immediate disruption of feed consumption and a 10-day pause in production when such incomplete diets were force-fed for only 5 days. Although feed consumption was not affected by the omission of glutamic acid, normal egg production could not be maintained. Tyrosine could be replaced by phenylalanine only with difficulty. The hen does not require glycine for egg production. This is in contrast to the need of the growing chick for this amino acid.

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