Characterization of Lipopolysaccharides Isolated from Agrobacterium tumefaciens

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Abstract
SUMMARY: Lipopolysaccharide (LPS) was isolated from two strains of Agrobacterium tumefaciens and analysed. It contained phosphate, hexose, hexosamine, fatty acids and 2-keto-3-deoxyoc-tonic acid. Of the compounds commonly found in LPS, heptose and galactosamine were absent. The fatty acid composition of the agrobacterial LPS is unique, since only 3-hydroxy-tetradecanoic acid and 3-hydroxyhexadecanoic acid were significant components. 3-Hydroxyhexadecanoic acid was linked via an amide bond to the carbohydrate backbone. Non-hydroxylated fatty acids were present only as impurities (< 3%).

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