METABOLISM OF GLYCOSAMINOGLYCANS OF CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS ALTERED DURING SUBCULTURE

Google Scholar
  • 1 January 1983
    • journal article
    • research article
    • Vol. 64  (2) , 156-165
Abstract
Changes in the metabolism of glycosaminoglycans of cultured smooth muscle cells from the rat aorta during subculture were observed. The primary culture was achieved by the enzymatic dispersion method. The metabolism of glycosaminoglycans in smooth muscle cells was estimated by measuring the incorporation rate of D-[1-14C] glucosamine and [35S]sulfate. Smooth muscle cells were harvested by trypsinization, and glycosaminoglycans were separately extracted and purified from trypsin digest and cells. In the cells of the stationary phase of primary culture, the incorporation of both D-[1-14C]glucosamine and [35S] sulfate into dermatan sulfate was greater than that into heparan sulfate. However, in the trypsin digest, the incorporation of D-[1-14C] glucosamine and [35S] sulfate into dermatan sulfate was less than and equal to that into heparan sulfate. In both the cells and the trypsin digest, the incorporation of D-[1-14C]glucosamine and [35S] sulfate into heparan sulfate decreased and that into dermatan sulfate increased with increase in the number of passages. There evidently is a development of serial modulation in the metabolism of glycosaminoglycans in the cultured smooth muscle cells, in the early passage of subculture and that such should be taken into consideration when analyzing the observations.