Dimorphic primers derived from intron 1 for use in the molecular typing of HLA‐B alleles

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Abstract
We have identified a dimorphic site in intron 1 of the HLA‐B gene. Oligotyping was performed on about 3000 samples using primers derived from this dimorphic site in combination with a locus‐specific primer derived from intron 3. The distribution of B‐alleles bearing each of the dimorphic sequences was approximately equal. These primers were mutually exclusive and yielded approximately 50% of the heterozygous samples as apparently homozygous in PCR products. Intermediate and almost high‐resolution oligotyping of HLA‐B alleles was achieved using 35 and 63 hybridization probes, respectively. This dimorphic site will provide a useful tool for otherPCR‐based HLA‐B typing approaches