Precursor-product relationship between the 26-kDa and 18-kDa fragments formed by iodination of human thyroglobulin
- 17 September 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 175 (1) , 140-146
- https://doi.org/10.1016/0014-5793(84)80587-6
Abstract
At moderate iodination levels (20 iodine at atoms/molecule), human thyroglobulin (hTgb) produces after reduction a thyroxinyl-peptide of 26 kDa which represents the N-terminal part of the protein. At higher iodination levels, the 26-kDa peptide is accompanied by another T4-containing peptide of 18 kDa. A precursor-product relationship between the 26- and 18-kDa fragments was demonstrated by the study of the tryptic fragments of both hormonopeptides. In addition, comparison with the protein sequence deduced from the nucleotide sequence of the 5'-end of hTgb mRNA demonstrated that the N-terminal region of Htgb from which are issued the 26-kDa peptide and its 18-kDa derivative is especially sensitive to proteolysis. This character is possibly related with a facilitated release of thyroid hormones in vivoKeywords
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