?-Adrenoceptors in the extraorbital lacrimal gland of the Syrian hamster characterization with [125I]-iodopindolol and evidence of sexual dimorphism

Publisher Website
Google Scholar
Abstract
Saturation and competition experiments with the radiolabeled β-adrenergic antagonist (−)-[125I]-iodopindolol were used to characterize β-adrenoceptor density (Bmax) and receptor affinity in the extraorbital lacrimal gland of male and female Syrian hamsters. Specific binding to the receptor was saturable. Scatchard analysis of saturation isotherms revealed a single population of receptor sites. Male glands had a significantly higher Bmax (38.9±5.0 vs. 23.3±2.1 fmole/mg protein, ¯x±SEM, p< 0.02) and receptor affinity (expressed in a lower dissociation constant Kd: 0.065±0.013 vs. 0.120±0.015 nM, p2-subtype.