Hydrolysis of corn oil by lipase fromHelminthosporium maydis race T

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Abstract
Corn oil was used as the sole carbon source for growth ofHelminthosporium maydis Race T (NRRL 5128) in shaken flasks at 28 C. Oil recovered by hexane extraction from culture medium and mycelia at 2‐day intervals was analyzed by thin layer chromatography and gas chromatography. Triglyceride content of the oil was reduced, whereas free fatty acid, monoglyceride, and diglyceride contents increased as a result ofHelminthosporium maydis growth. Free sterol and steryl ester contents were unaffected. Lipase production was demonstrated byHelminthosporium maydis cells grown on corn oil, but the enzyme was not detected in cells grown on sucrose.