A New Method for the Detection of Organophosphorus Pesticides byin situFluorometry on Thin-Layer Chromatograms

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Abstract
A new technique for rendering certain organophosphorus pesticides fluorescent has been developed which requires heating the chromatoplate at an optimum temperature for a definite period of time. Some of the pesticides that give positive results are: menazon, coumaphos, azinphosmethyl, and MARETIN. Menazon and coumaphos are of particular interest since both compounds are difficult to detect by gas chromatography. Fluorescence is produced from other organophosphorus pesticides by spraying the chromatogram with a strong base prior to heating. The principal advantage of the technique is that the fluorescence is generated in situ without the use of a spray reagent and fluorescence quantitation should thus be greatly facilitated. The technique is simple, rapid, and affords excellent sensitivity. Visual detection limits ranging from 0.001 mcg to 0.1 mcg have been obtained.

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