Purification and Properties of a Fructooligosaccharide-producing β-Fructofuranosidase fromAspergillus nigerATCC 20611

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Abstract
A fructooligosaccharide-producing β-fruetofuranosidase was purified from cells of Aspergillus niger ATCC 20611. The molecular weight was 340,000 by gel filtration. The optimum pH of the enzyme was 5.0 ~ 6.0 and the optimum temperature was 50~60°C. The enzyme was rendered inactive by 1 mM Hg2+ and the Km value for sucrose was 0.29 m. The enzyme catalyzed an almost exclusively fructosyl transfer reaction in a 50% sucrose solution to produce a mixture of fructooligosaccharides and glucose, but both fructosyl transfer and hydrolytic action were observed in a 0.5 % solution. The β-fructofuranosidase showed a high regiospecificity to transfer the fructosyl moiety for the 1-OH group of terminal fructofuranosides.

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