The Effect of Asparagine on the Growth of a Gram-Positive Coccus

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Abstract
A Pediococcus sp., unusual in that it needed reducing conditions for growth, would not grow in a medium based on acid-hydrolysed casein but grew when asparagine was added. Neither glutamine nor aspartic acid + an ammonium salt satisfied this requirement. When the acid-hydrolysed casein was replaced by a mixture of amino acids, the omission of asparagine caused a decrease in growth in the presence of aspartic acid + an ammonium salt. No growth occurred in the absence of both aspartic acid and asparagine.