Xenopus oocytes can synthesise but do not secrete the Z variant of human α1‐antitrypsin
- 12 March 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 168 (1) , 84-88
- https://doi.org/10.1016/0014-5793(84)80211-2
Abstract
Human liver mRNA was prepared from a patient homozygous for α1-antitrypsin deficiency (PiZZ) and from a normal subject (PiMM). Both liver RNAs were microinjected into Xenopus oocytes and α1-antitrypsin identified by immunoprecipitation. The normal M variant of α1-antitrypsin is synthesised and secreted by Xenopus oocytes, the abnormal Z protein is not secreted and an intracellular form accumulates in the oocytes. In the presence of tunicamycin an unglycosylated form of M α1-antitrypsin appears in the incubation medium but no corresponding unglycosylated version of the Z protein is secreted.Keywords
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