The release of lipoproteins by rat liver slices

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Abstract
The release of the lipid and protein moieties of lipoproteins by liver slices from rats charged in vivo with palmitate-9,10-3H and leucine-1-14C was followed. The replacement of Ringer solution as the incubation medium by serum resulted in an increase in the release of the labelled lipid moiety but had no effect on the radioactivity of the protein moiety. Analysis of the lipoproteins indicated that serum had no effect on the radioactivity of the neutral lipid in the lipoprotein of d < 1.063 or β-lipoproteins prepared by ultracentrifugation or heparin precipitation, respectively. However, the lipoprotein of d < 1.063 released into serum contained considerable amounts of labelled phospholipids and fatty acids which were not present in the β-lipoprotein precipitated by heparin or in the low density lipoproteins prepared by either procedure following incubation in Ringer solution. Following incubation of the slices in serum, but not in Ringer solution, the lipoprotein having d > 1.063 and the supernatant of the heparin precipitate contained considerable amounts of labelled phospholipid. It is concluded that serum may contain a lipoprotein capable of combining with liver phospholipids and that ultracentrifugation results in the separation of low density lipoproteins containing excess lipid.

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