The G‐protein‐coupled CCK2 receptor associates with phospholipase Cγ1
Open Access
- 19 May 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 568 (1-3) , 89-93
- https://doi.org/10.1016/j.febslet.2004.05.012
Abstract
In ElasCCK2 transgenic mice expressing cholecystokinin (CCK2) receptor in acinar cells, pancreatic phenotypic alterations and preneoplastic lesions are observed. We determined whether activation of phospholipase C gamma1 (PLCγ1), known to contribute to the tumorigenesis pathophysiology, could take place as a new signaling pathway induced by the CCK2 receptor. Overexpression and activation of the PLCγ1 in response to gastrin was observed in acinar cells. The possibility that the C‐terminal tyrosine 438 of the CCK2 receptor associates with the SH2 domains of PLCγ1 was examined. A specific interaction was demonstrated using surface plasmon resonance, confirmed in a cellular system and by molecular modeling.Keywords
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