EFFECTS OF ESTRADIOL ON HEMATOPOIETIC AND MARROW ADHERENT CELLS OF DOGS

  • 1 April 1986
    • journal article
    • research article
    • Vol. 47  (4) , 906-909
Abstract
Hematopoietic and marrow adherent cells were examined in 5 ovariohysterectomized dogs treated once with 1.5 mg of 17.beta.-estradiol cyclopentylpropionate/kg of body weight. All dogs initially developed thrombocytopenia and neutrophilic leukocytosis, with megakaryocytopenia and an increased myeloid/erythroid ratio of the marrow. Later, 2 dogs became thrombocytopenic and neutropenic, with megakaryocytopenia, decreased myeloid/erythroid ratio, and increased concentration of plasma cells and mast cells in the marrow. The concentration of marrow granulocyte-macrophage colony-forming units (CFU-GM) was decreased at 2 and 3 weeks in all dogs after estradiol treatment. The concentration of marrow fibroblast CFU-F was decreased in all estradiol-treated dogs at 1 and 2 weeks. The ability of marrow adherent cells to support CFU-GM colony formation was unchanged in estradiol-treated dogs. Estradiol sulfate at final concentrations of 10-8 to 10-4 M did not affect the in vitro growth of CFU-GM and CFU-F, or the hematopoietic ability of marow adherent cells from nontreated dogs.