Flow cytometric analysis ofActinidiaspecies

Publisher Website
Google Scholar
Abstract
Nuclei were extracted from shoot apices of Actinidia species, treated with ribonuclease, stained with propidium iodide, and then analysed by flow cytometry. Nuclei from shoots and from callus of in vitro cultures were also analysed. Diploid, tetraploid, hexaploid species, and hybrids between diploid and hexaploid could be accurately identified. No mixoploidy or aneuploidy was found in shoots from potted plants or from shoots of tissue-cultured plants following repeated subculturing. However, two cytotypes (DNA dodecaploid) of A. deliciosa were identified in plant populations derived by adventitious organogenesis from callus. The cytotypes were stable over a 2 year period. The nuclear DNA content of Actinidia species was determined by co-chopping shoot apices with Hordeum, Vigna, and Zea standards. Nuclear DNA contents were 1.53, 3.09, and 4.43 pg DNA per 2 C nucleus for A. chinensis var. chinensis, A. arguta var. arguta, and A. deliciosa var. deliciosa, respectively.