Autolysis as an Aid to Cysticercoid Counting in Experimental Hymenolepis nana Infections

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Abstract
A modified technique is described which permits rapid counting of cysticercoids or newly emerged worms of the dwarf mouse tapeworm, Hymenolepis nana, in the small intestine of its host. The intestine is removed 90-100 hours after experimental feeding of tapeworm eggs. The gut is refrigerated in tap or distilled water for 48 hours, allowing autolytic and bacterial breakdown of the mucus and intestinal contents. The intestine is then slit, carefully washed, and examined under a dissecting microscope. Infected villi can be observed and counted. Previously infected villi can be identified by the remnant of cysticercoid membranes remaining in the villi, and newly emerged worms are usually found intact and attached to the mucosal wall which has been cleared of mucus and other obscuring material.

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