Effect of Polyamines on Globin Synthesis in a Rabbit Reticulocyte Polyamine-Free Protein Synthetic System1
- 1 February 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 105 (2) , 164-167
- https://doi.org/10.1093/oxfordjournals.jbchem.a122633
Abstract
A polyamine-free protein synthetic system, established from components of rabbit reticulocytes, consisted of globin mRNA, salt-washed ribosomes, partially purified initiation factors, and pH 5 enzymes. Spermidine added to this system not only lowered the optimal magnesium concentration required for globin synthesis, but it also stimulated the globin synthesis 6- to 8-fold. The optimal spermidine concentration was 0.4 to 0.6 mM, a concentration similar to that in intact rabbit reticulocytes. The ratio of a to α and β globin chains synthesized in the presence of spermidine and Mg2+ was approximately 1.0, while the ratio in the presence of only Mg2+ was approximately 1.5. Formation of methionyl puromycin was stimulated 4- to 6-fold by spermidine. This indicates that the stimulation is mainly at the level of initiation.Keywords
This publication has 0 references indexed in Scilit: