Genome-wide comparison of differences in the integration sites of interspersed repeats between closely related genomes
- 15 July 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 30 (14) , 71e-71
- https://doi.org/10.1093/nar/gnf071
Abstract
A technique for genome-wide detection of differences in the integration site positions of interspersed repeats in related genomes (DiffIR) is described. The technique is based on a whole- genome selective PCR amplification of the repeats' flanking regions followed by a differential hybridization screening of the arrayed library of the selected amplicons. The technique was successfully applied to the comparison of the integration sites in the human and chimpanzee genomes, allowing us to discover 11 new human-specific integrations of human endogenous retrovirus, K family (HML-2) long terminal repeats.Keywords
This publication has 25 references indexed in Scilit:
- Large-scale analysis of the Alu Ya5 and Yb8 subfamilies and their contribution to human genomic diversityJournal of Molecular Biology, 2001
- Full-sized HERV-K (HML-2) human endogenous retroviral LTR sequences on human chromosome 21: map locations and evolutionary historyGene, 2001
- Molecular description of three macro-deletions and an Alu–Alu recombination-mediated duplication in the HPRT gene in four patients with Lesch-Nyhan diseaseMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2001
- Identification of a Genomic Island Present in the Majority of Pathogenic Isolates of Pseudomonas aeruginosaJournal of Bacteriology, 2001
- Initial sequencing and analysis of the human genomeNature, 2001
- Reading between the LINEs: Human Genomic Variation Induced by LINE-1 RetrotranspositionGenome Research, 2000
- A new exon created by intronic insertion of a rearranged LINE-1 element as the cause of chronic granulomatous diseaseEuropean Journal of Human Genetics, 2000
- L1 Retrotransposons Shape the Mammalian GenomeScience, 2000
- Human L1 retrotransposition: insights and peculiarities learned from a cultured cell retrotransposition assayPublished by Springer Nature ,2000
- Many human endogenous retrovirus K (HERV-K) proviruses are unique to humansCurrent Biology, 1999