Synchronization of Human Diploid Cells by Fluorodeoxyuridine. The First Ten Minutes of Synthesis in Female Cells2

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Abstract
Partial synchronization of human fetal diploid cells was accomplished with 5-fluoro-2′-deoxyuridine (FUDR) followed by addition of exogenous thymidine (TDR). A maximum of 75% of cells were in DNA synthesis (S) following this synchronization procedure, as compared to 30% for asynchronous cells. Optimal synchronization was obtained with 0.1 μg/ml of FUDR for 20 hours. Shortening of the S period by FUDR is suggested. The partial synchrony produced by FUDR block and TDR reversal was lost before completion of a cell cycle. Synchronization by FUDR was used to study the chromosome labeling patterns in human female fetal-diploid cells exposed to 3H-TDR during the first 10 minutes of S. Approximately 60% of metaphases collected at next division after labeling contained an unlabeled C group chromosome. It ranged in total length between the longer and the shorter chromosomes of the C group and was presumed to be an unlabeled X chromosome. No other chromosomes were consistently unlabeled during the first 10 minutes of S.