Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.
- 1 October 1982
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 1 (5)
- https://doi.org/10.1007/bf00270237
Abstract
Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars (“Loras” and “Tower”) of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. They developed to mature embryos when the auxin was reduced or omitted.Keywords
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