Abstract
A total of 20 spp. in the D. montium subgroup were cytologically examined. All species show a similar basic pattern of metaphase karyotype. The most extensive variations in metaphase chromosome configurations were observed in the Y and 4th (dot) chromosomes, while the X chromosome is slightly variable. Interspecific karyotype differentiation is largely due to the acquisition of different amounts of heterochromatin. [The species studied were D. auraria, D. baimaii, D. barbarae, D. birchii, D. bocki, D. dominicana, D. jambulina, D. kikkawai, D. lacteicornis, D. leontia, D. lini, D. mayri, D. nikananu, D. orosa, D. pennae, D. pseudomayri, D. punjabiensis, D. rufa, D. seguyi and D. serrata].