EXPRESSION OF THE M-CSF (CSF-1) GENE BY HUMAN-MONOCYTES

  • 1 May 1987
    • journal article
    • research article
    • Vol. 69  (5) , 1409-1413
Abstract
Monocyte colony-stimulating factor (M-CSF CSF-1) is a macrophage lineage-specific growth factor. Northern blot analysis using a human M-CSF cDNA probe and a specific bioassay for human M-CSF were used to investigate the cellular sources of M-CSF. Expression of the M-CSF gene was induced in blood mononuclear cells stimulated by phorbol myristate acetate (PMA) or .gamma.-interferon. When mononuclear cells were fractionated into highly purified populations of T cells and monocytes, M-CSF transcripts were detected predominantly in monocytes. Further, monocytes stimulated with PMA released an M-CSF-like activity. These results demonstrate that M-CSF can be produced by activated cells of the macrophage lineage.