An Enzyme-Linked Immunosorbent Blocking Assay for Comparing Closely Related Virus Isolates

Publisher Website
Google Scholar
Abstract
Ouchterlony double diffusion comparison of tomato ringspot virus (TmRSV) isolated from apple (A), blueberry (B), geranium (G) and cymbidium orchid (O) showed that all 4 isolates had at least 1 antigenic determinant in common, and A- and B-TmRSV had a least 1 additional antigenic determinant in common, which was not shared by TmRSV-G or -O. An enzyme-linked immunosorbent blocking assay (EIBA), which compares the relative abilities of excess unlabeled heterologous and homologous antigen to block the binding of enzyme-labeled antigen to its immobilized homologous IgG, was developed to more closely scrutinize the serological relationships. EIBA revealed additional antigenic differences among the 4 TmRSV isolates not detected by double diffusion tests.

This publication has 0 references indexed in Scilit: