The agar contact replica technique after isoelectric focusing as a screening method for the detection of enzyme variants
- 1 February 1982
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 3 (3) , 142-145
- https://doi.org/10.1002/elps.1150030305
Abstract
Electrofocusing has been adapted in order to make screening experiments for the detection of enzyme variants more efficient. The principle of the new technique consists in making agar copies of the polyacrylamide gel by contact; the original gel and the agar copies are then developed for different enzyme systems. By doing so, the number of enzymes,i.e.loci, per test animal screened after a single electrofocusing run is increased. This method has proved to be feasible for the detection of mouse variants with altered enzyme banding patterns due to parental treatment with chemical mutagens.This publication has 8 references indexed in Scilit:
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