Genetic Identification of Staphylococcus aureus by Polymerase Chain Reaction Using Single‐Base‐Pair Mismatch in 16S Ribosomal RNA Gene
Open Access
- 14 November 1995
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 39 (11) , 839-844
- https://doi.org/10.1111/j.1348-0421.1995.tb03280.x
Abstract
Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single‐base‐pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureus without requiring additional analytical techniques. Twenty‐eight staphylococcal and non‐staphylococcal strains were tested to verify the specificity of the assay, and only S. aureus strains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus.Keywords
This publication has 18 references indexed in Scilit:
- The interrelationships of all major groups of Platyhelminthes: phylogenetic evidence from morphology and moleculesBiological Journal of the Linnean Society, 1999
- PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluidJournal of Clinical Microbiology, 1994
- Update on clinical significance of coagulase-negative staphylococciClinical Microbiology Reviews, 1994
- The Identification of Uncultured Microbial PathogensThe Journal of Infectious Diseases, 1993
- Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNAMolecular and Cellular Probes, 1993
- Prevalence of Bacterial Pathogens and Antimicrobial Susceptibility: A Multicenter Study in Akita PrefectureKansenshogaku Zasshi, 1993
- Genetic disease detection and DNA amplification using cloned thermostable ligase.Proceedings of the National Academy of Sciences, 1991
- Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsiesDiagnostic Microbiology and Infectious Disease, 1990
- Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)Nucleic Acids Research, 1989
- The number of rRNA genes in Escherichia coliFEBS Letters, 1977