Dye binding mechanisms in G‐banding of chromosomes
- 2 August 1980
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 119 (3) , 397-406
- https://doi.org/10.1111/j.1365-2818.1980.tb04111.x
Abstract
The mechanisms whereby Giemsa is bound to chromosomes to produce G-banding patterns have been studied. The magenta colour produced in chromosomes by Giemsa staining appears to be due to the same 2:1 thiazine-eosin compound that precipitates from Giemsa solutions. This precipitate is formed in chromosomes in regions in which DNA phosphates occur at the correct distance apart to bind two thiazine molecules, which are subsequently able to bind to the same eosin molecule. Banding appears to be produced as a result of this precipitation occurring preferentially in hydrophobic regions of chromosomes.Keywords
This publication has 13 references indexed in Scilit:
- The Mechanism of Action of Antitumor Platinum CompoundsProgress in Nucleic Acid Research and Molecular Biology, 1979
- Changes in elemental composition of human chromosomes during a G-banding (ASG) and a C-banding (BSG) procedureJournal of Molecular Histology, 1978
- A comparison of methods for blocking staining of nucleic acidsActa Histochemica, 1978
- Differentiation of DNA . Platinum Complexes by Fluorescence. The Use of an Intercalating Dye as a ProbeEuropean Journal of Biochemistry, 1977
- Mechanisms of chromosome bandingChromosoma, 1975
- Involvement of protein disulphides and sulphydryls in chromosome bandingExperimental Cell Research, 1974
- Mechanisms involved in the banding of chromosomes with quinacrine and Giemsa: II. The interaction of the dyes with the chromosomal componentsExperimental Cell Research, 1973
- New Technique for Distinguishing between Human ChromosomesNature New Biology, 1971
- Zirconium (IV) oxidechloride as a blocking agent for staining with toluidine blue in the pH interval 1.42–4.58Cells Tissues Organs, 1968