Immunogenic Correlation between Cross-Reacting Material (CRM197) Produced by a Mutant of Corynebacterium diphtheriae and Diphtheria Toxoid
- 1 November 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 142 (5) , 716-724
- https://doi.org/10.1093/infdis/142.5.716
Abstract
The in vivo immunizing potency of diphtheria toxoid and formalin-treated cross-reacting material (CRM197, a nontoxic mutant protein) was compared in guinea pigs. Major antigenic differences between the 2 untreated proteins were also tested in rats. Diphtheria toxoid and CRM197 were equally effective immunogens, but only if the latter was treated with formalin in the same concentration (0.7% vol/vol) as that of the toxoid. Formalin treatment rendered the antigens more resistant to enzymatic proteolysis by trypsin in vitro.This publication has 2 references indexed in Scilit:
- Demonstration of diphtheria toxin receptors on surface membranes from both toxin-sensitive and toxin-resistant species.Journal of Biological Chemistry, 1978
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951