Quorum quenching enzyme activity is widely conserved in the sera of mammalian species
Open Access
- 13 June 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (17) , 3713-3717
- https://doi.org/10.1016/j.febslet.2005.05.060
Abstract
Acyl‐homoserine lactone (AHL) quorum sensing signals play a key role in synchronizing virulence gene expression in Pseudomonas aeruginosa, which could cause fatal bloodstream infections. We showed that AHL inactivation activity, albeit with variable efficiency, was conserved in the serum samples of all the 6 tested mammalian animals. High‐performance liquid chromatography and mass spectrometry analyses revealed that mammalian sera had a lactonase‐like enzyme(s), which hydrolyzed the lactone ring of AHL to produce acyl homoserine, with enzyme properties reminiscent of paraoxonases (PONs). We further showed that the animal cell lines expressing three mouse PON genes, respectively, displayed strong AHL degradation activities.Keywords
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