Production and Characterization of Monoclonal Antibodies to the Major Peripheral Myelin Glycoprotein P0
- 1 April 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 54 (4) , 1110-1114
- https://doi.org/10.1111/j.1471-4159.1990.tb01936.x
Abstract
Several monoclonal antibodies were generated against the major glycoprotein P0 of human peripheral nervous system myelin. Antibodies were selected for their reactivity with P0 in Western blots. The antibodies were of the immunoglobulin G subclass and reacted with the glycopeptidase F-treated P0, indicating that the reactive epitope resides in the protein backbone. In fresh frozen and paraffin-embedded sections of central and peripheral nervous system of rat and human P0 antibody 592 reacted with myelin sheaths of peripheral, but not central, nervous system.Keywords
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