Use of the fluorescence technique as an absolute method for obtaining mean relaxation times of globular proteins

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Abstract
Measurements of excited lifetime and of degree of polarization have been combined to yield values for the mean relaxation time of fluorescent conjugates of bovine serum albumin, egg albumin, and lysozyme. Evidence has been presented for the validity of relaxation times thus computed and for the absence of contributions from nonrandomness of fluorescent residue orientation and from internal degrees of rotational freedom.