Biochemical and Histochemical Studies of Aerobic Oxidative Metabolism of Oral Tissues. II. Enzymatic Dissection of Gingival and Tongue Epithelia from Connective Tissues

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Abstract
The gingival and tongue epithelium were separated from underlying connective tissues, at low temperatures (0[degree]to5[degree]C) in gingiva of human and dog, and in tongue of dog, rabbit and rat,by NH4CNS, KCNS, pepsin, collagenase, pronase, papain, elastase, pan-protease, trypsin and trypsin-hyaluroni-dase. Best separations were obtained with CNS compounds, pepsin and collagenase. The other enzymes produced a disruption of epithelial cells in extremely low concentrations. There were species differences in response of gingival and tongue tissues to the action of the agents. There were also differential influences on staining properties of treated tissues. Both frozen sections and intact biopsy specimens were used. Metabolic (aerobic oxidative) activity was studied. The CNS compounds inhibited cytochrome oxidase activity, whereas pepsin and collagenase did not. Results of matabolic studies are given.