Determination of promethazine in serum by liquid chromatography.
Open Access
- 1 February 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 27 (2) , 253-255
- https://doi.org/10.1093/clinchem/27.2.253
Abstract
We describe a procedure for the liquid-chromatographic determination of promethazine in low-nanogram concentrations in serum. Triflupromazine is used as the internal standard. The method is based on a single extraction of promethazine from serum with hexane and subsequent derivatization with trichloroethyl chloroformate. Analytical recovery of promethazine is about 90%. The lower limit of detection is 1 microgram/L when a 2.0-mL aliquot of serum is assayed. Our data illustrate the practicability of the method for bioavailability studies after oral or rectal administration of promethazine hydrochloride.This publication has 1 reference indexed in Scilit:
- The Detection of Some Basic Drugs and Their Major Metabolites Using Gas-Liquid ChromatographyClinical Toxicology, 1979