Improved Immunoperoxidase Stain on Frozen Sections: An Avidin-Biotin-Peroxidase Complex (ABC) Technique
- 1 June 1982
- journal article
- research article
- Published by Maney Publishing in Journal of Histotechnology
- Vol. 5 (2) , 61-63
- https://doi.org/10.1179/014788882794580399
Abstract
A modified Avidin-Biotin-Peroxidase complex (ABC) technique is described for use on frozen tissue sections and compared with the PAP technique. Increasing the incubation temperature and decreasing the incubation time resulted in (1) increased sensitivity with less background staining, (2) reduced turnaround time and (3) decreased cost with the modified ABC technique.Keywords
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