Improved Luciferase Tagging System for Listeria monocytogenes Allows Real-Time Monitoring In Vivo and In Vitro
Open Access
- 1 May 2007
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (9) , 3091-3094
- https://doi.org/10.1128/aem.02940-06
Abstract
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.Keywords
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