BENZIDINE POSITIVE MULTICENTRIC EOSINOPHILIC COLONIES IN HUMAN-BLOOD MIMICKING ERYTHROID BURSTS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (1) , 64-70
Abstract
When normal human mononuclear blood cells are cultured in plasma clot or methylcellulose in the presence of erythropoietin, 2 types of benzidine positive multicentric colonies can be recognized on light microscopy at high magnification: one containing diffusely stained cells with the morphology of erythroblasts (erythroid bursts), and another containing diffusely and/or granular stained benzidine positive cells with a morphology of neutrophils/macrophages. Based on their cytochemical properties, lack of inhibition of the benzidine reaction by potassium cyanide, and strong granular staining with luxol fast blue and alkaline eosin, the cells from the latter are identified as eosinophils.