The Mechanism of Histamine Binding to Heparin

Abstract
The interaction between polyanions and cations is electrostatic in nature, since it is characterized by its dependence on pH and ionic strenth. Many of the properties of heparin, including its anticoagulant activity and histamine binding capacity, are due to its ability to bind to organic and inorganic ions or molecules. The ability is related to the type, number and distribution of ionizable groups on the heparin molecule, coupled with a size effect. Pulse radiolysis and the spectroscopic method were used to evaluate the limiting concentration for the destruction of a heparin-MB+ [methylene blue] complex by histamine and N-acetyl histamine over the pH range 3-11 to investigate the mechanism of histamine binding to heparin.

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