2'-Deoxy-2'-halonucleotides as alternate substrates and mechanism-based inactivators of Lactobacillus leichmannii ribonucleotide reductase

Publisher Website
Google Scholar
Abstract
The interaction of the ribonucleotide-triphosphate reductase of Lactobacillus leichmannii with various 2''-halogenated ribo- and arabinonucleoside triphosphates has been investigated. All analogues examined acted as mechanism-based inactivators of the enzyme, producing base, triphosphate, and halide. In all cases, the inactive enzyme had developed the distinctive chromophore at 320 nm that is characteristic of enzyme inactivated by 2-methylene-3(2H)-furanone. The striking similarities between these results and those previously reported for the inactivation of this enzyme by 2''-chloro-2''-deoxyuridine triphosphate suggest a common reaction path for all 2''-halonucleotides. In the pyrimidine series, it was found that 2''-fluoro- and 2''-chloronucleotides partitioned between inactivation and formation of the normal reduction product 2''-deoxynucleotide. Normal reduction predominated with 2''-fluoronucleotides, whereas it was a minor pathway for 2''-chloro-2''deoxyuridine triphosphate. With 2''-chloro-2''-deoxyuridine triphosphate, the relative partitioning between the two modes was pH dependent: the amount of 2''-deoxyuridine triphosphate formed increased 2.8-fold upon changing from pH 6.1 to pH 8.3. The ability of 2''-arabinohalonucleotides to inactive ribonucleotide reductase and the variation of partitioning of the pyrmidine analogues with leaving group and reaction pH are consistent with our radical cation hypothesis and support the proposal that the difference between normal catalysis and inactivation is related to the protonation state of the reductase.