Typing of the short tandem repeat D8S347 locus with different fluorescence markers

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Abstract
The short tandem repeat (STR) locus D8S347 was analyzed by capillary electrophoresis. Sequencing data and a population study of 203 individuals from a southwestern German population are presented. We detected 12 different alleles, 340–388 bp in length, and found 40 different genotypes. The heterozygosity index was 85.7%. Futhermore, we investigated the consequences of different fluorescent dyes, namely 6‐FAM, HEX, and ROX, on the ABI‐calculated fragment sizes of defined (i.e., sequenced) alleles (348–376 bp in length). 6‐FAM‐labeled fragments appear to be smaller than the corresponding HEX‐or ROX‐labeled fragments. On average, 6‐FAM‐labeled fragments differ by 3.52 bp from the sequencing data, HEX‐labeled ones by 2.04 bp, and ROX‐labeled ones by 1.42 bp. Generally, small alleles differ less from the expected sequencing data than larger ones.