STUDY OF CELL DOSE AND STORAGE TIME ON ENGRAFTMENT OF CRYOPRESERVED AUTOLOGOUS BONE MARROW IN A CANINE MODEL

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Abstract
The i.v. infusion of cryopreserved autologous bone marrow can avert the otherwise lethal myelosuppression of total-body irradiation in rodents, dogs, and nonhuman primates. We have developed a canine model in an effort to study factors which may influence the engraftment of cryopreserved autologous bone marrow. Dogs were subjected to 1,000 rad of total-body irradiation at 9 rad/min and then supported with fluids and antibiotics alone or with an infusion of their own cryopreserved bone marrow, which had been removed and stored in 10% dimethyl sulfoxide in the vapor phase of liquid nitrogen prior to irradiation.