Synthesis of 125I-Labeled Oligonucleotides from Tributylstannylbenzamide Conjugates
- 1 March 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 8 (2) , 238-243
- https://doi.org/10.1021/bc970016n
Abstract
A rapid and efficient method for the synthesis of 125I-labeled oligodeoxynucleotides ([125I]ODNs) is described. The key intermediates are tributylstannylbenzamide-modified ODNs (Sn-ODNs). Reaction conditions are described for the preparation of 5'-modified Sn-ODNs. Treatment with NaI and chloramine T gave conversion to the desired I-ODN, which was easily isolated by reversed phase chromatography. Thermal denaturation (Tm) studies showed that hybridization properties were not disturbed by the 4-iodobenzamide modification. An [125I]ODN was prepared and characterized by hybridization to 32P-labeled DNA targets. Sequence specific cleavage of the target DNA strand by 125I was measured.Keywords
This publication has 12 references indexed in Scilit:
- Synthesis and use of 4‐hydroxyphenyl derivatized phosphoramidites in the selective radioiodination of oligonucleotide probesJournal of Labelled Compounds and Radiopharmaceuticals, 1993
- Facile preparation of nuclease resistant 3′ modified oligodeoxynucleotidesNucleic Acids Research, 1993
- Radiohalogenation of proteins: An overview of radionuclides, labeling methods and reagents for conjugate labelingBioconjugate Chemistry, 1992
- Site-specific incorporation of [125I]iododeoxyuridine into DNAInternational Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes, 1992
- Acridine- and cholesterol-derivatized solid supports for improved synthesis of 3'-modified oligonucleotidesBioconjugate Chemistry, 1991
- Development of sensitive radioiodinated anti-sense oligonucleotide probes by conjugation techniquesBioconjugate Chemistry, 1991
- Range of Radiochemical Damage to DNA with Decay of Iodine-125Science, 1981
- 5-125I-iododeoxyuridine as prototype for radionuclide therapy with Auger emittersNature, 1977
- Iodination of nucleic acids in vitroBiochemistry, 1971
- Oligonucleotide interactions. III. Circular dichroism studies of the conformation of deoxyoligonucleolidesBiopolymers, 1970