The Properties of Human Fibrinogen Coagulum—Preliminary Report

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Abstract
The effects of various factors on fibrinogen clots and their tensile strength was investigated in an effort to determine optimal conditions in the use of these clots for the operative removal of renal calculi. The time necessary to obtain clotting of fibrinogen soln. by clotting globulin (Lederle) varies with the conc. of the latter substance. 2% clotting globulin produces coagulation in 30 secs. and was chosen as the most suitable conc. Tensile strength of the coagulum increases rapidly during the first 5 mins. after clotting and, at room temp., increases more slowly during the next half hr. At 37[degree]C, however, maximum tensile strength of the coagulum is attained 5 mins. after coagulation. Fibrinogen soln. may be maintained at 37[degree]C. for 1 hr. without decreasing the tensile strength of its coagulum. Incorporation of human blood and urine within the coagulum causes only a slight decrease in tensile strength and apparently they act only as diluting factors. Na sulfa-thiazole, in 0.2% conc. does not affect tensile strength of the clot, but does not sterilize fibrinogen solns. under the conditions necessary for preservation of this material. The coagulum is not rapidly disintegrated by certain known proteolytic agents; it is disintegrated within 18-24 hrs. by normal or infected human urine. Certain detergents in small amts. may be incorporated in the coagulum without decreasing its tensile strength. Production of human fibrinogen coagula within the pelvis of the human kidney is without apparent ill effect.

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