The Reversible and Irreversible Toxic Effects of Antihela Cell Rabbit Serum upon Human Cell Lines in Tissue Culture
Open Access
- 1 February 1958
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 80 (2) , 100-105
- https://doi.org/10.4049/jimmunol.80.2.100
Abstract
Summary: Anti-HeLa cell rabbit serum may produce a variety of cytotoxic effects upon the following cell lines in tissue culture: HeLa, AU, T17, and T27. Irreversible cytotoxic changes are produced when anti-HeLa cell rabbit serum and a heat labile substance in normal guinea pig or rabbit serum are added to these cell lines. Reversible morphologic changes in human cell lines are produced when incubated for 2 hr with anti-HeLa cell rabbit serum. Marked depression of the growth rate of HeLa cells occurs when they are propagated in a medium containing anti-HeLa cell rabbit serum. The addition of complement destroys cultures of HeLa cells propagated for up to six months in an excess of anti-HeLa cell rabbit serum.Keywords
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