Serological identification of neoantigens on mouse fibroblasts which have undergone “spontaneous” malignant alterationin vitro

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Abstract
ST‐L1 is a cell line established from lung explants from a normal ST/a mouse. The ST‐L1 cells have undergone spontaneous malignant alteration in vitro. The cells were rejected after inoculation into syngeneic immunocompetent hosts, and a syngeneic humoral immuneresponse against the ST‐L1 cells has been detected. The specificity of this humoral response was investigated. The syngeneic response to ST‐L1 was characterized by indirect immunofluorescence tests and by immunoprecipitation of radiolabelled cells and of a C‐type virus produced by the cell lines. The specific anti‐ST‐L1 reactivities were found to be directed against the envelope glycoprotein of an endogenous C‐type virus expressed by the antigenic cell line.