The Effect of Methotrexate on in Vitro Immutoglobulin Production

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Abstract
The effect of Methotrexate (MTX) on pokeweed mitogen (PWM) induced immunoglobulin (Ig) production in vitro was studied over a reinge of 10-4 - 1012M MTX, using an enzyme-linked-immunosorbent assay (ELISA). MTX, at a concentration of 5 × 10-9 profoundly decreased all classes of Ig production, IgM > IgG > IgA. Plasma cell numbers, identified using polyclonal antihuman immunoglobulin, demonstrated a similar sensitivity to MTX. There was a paradoxical increase in IgM and IgG production at 5 × 1011M MTX. Clinical implications of these findings are discussed.

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