8-Hydroxydeoxyguanosine in dna Inhibits Restriction Endonuclease Digestion
- 1 February 1990
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 9 (2) , 223-233
- https://doi.org/10.1080/07328319008045134
Abstract
A DNA fragment containing 8-hydroxydeoxyguanosine was used to prime second strand synthesis of a recombinant pTZI9 phagemid single stranded DNA. The resultant double stranded closed circular product and also the annealed product of the single stranded DNA and the fragment were found to be resistant to cleavage by the restriction enzyme ApaLI in whose recognition sequence the 8-hydroxydeoxyguanosine had been inserted.This publication has 12 references indexed in Scilit:
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