A sensitive radioimmuno assay for thymine dimers
- 1 August 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 186 (4) , 475-477
- https://doi.org/10.1007/bf00337950
Abstract
Summary A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers \(\left( {\overline {TT} } \right)\) has been developed. The limit of detection of this method is 6×10-14 mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer \(\left( {\overline {UU} } \right)\) interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.
Keywords
This publication has 19 references indexed in Scilit:
- The development of a radioimmunoassay for the detection of photoproducts in mammalian cell DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Fluorescent-light-induced lethality and DNA repair in normal and xeroderma pigmentosum fibroblastsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- DNA Repair in Bacteria and Mammalian CellsAnnual Review of Biochemistry, 1979
- DNA Repair: New Clues to CarcinogenesisScience, 1978
- Repair deficient human disorders and cancerNature, 1978
- A SENSITIVE METHOD FOR MEASURING PYRIMIDINE DIMERS IN SITUPhotochemistry and Photobiology, 1977
- Preferential DNA Repair in Human CellsNature, 1974
- Immunological demonstration of the disappearance of pyrimidine dimers from nuclei of cultured human cellsExperimental Cell Research, 1972
- Methods for the assay of ultraviolet light-induced pyrimidine dimers in Saccharomyces cerevisiaeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Isolation and identification of the irradiation product of thymineBiochimica et Biophysica Acta, 1960