Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media
- 5 March 2009
- journal article
- research article
- Published by Springer Nature in Nature Protocols
- Vol. 4 (3) , 412-423
- https://doi.org/10.1038/nprot.2008.243
Abstract
We describe a simple and versatile scheme to prepare a series of poly(ethylene glycol)-based bidentate ligands that permit strong interactions with colloidal semiconductor nanocrystals (quantum dots, QDs) and gold nanoparticles (AuNPs) alike and promote their dispersion in aqueous solutions. These ligands are synthesized by coupling poly(ethylene glycol)s of various chain length to thioctic acid, followed by ring opening of the 1,2-dithiolane moiety to create a bidentate thiol anchoring group with enhanced affinity for CdSe-ZnS core-shell QDs. These ligands provide a straightforward means of preparing QDs and AuNPs that exhibit greater resistance to environmental changes, facilitating their effective use in bioassays and live cell imaging.Keywords
This publication has 41 references indexed in Scilit:
- In vivo cancer targeting and imaging with semiconductor quantum dotsNature Biotechnology, 2004
- Multiplexed Toxin Analysis Using Four Colors of Quantum Dot FluororeagentsAnalytical Chemistry, 2003
- Self-assembled nanoscale biosensors based on quantum dot FRET donorsNature Materials, 2003
- Long-term multiple color imaging of live cells using quantum dot bioconjugatesNature Biotechnology, 2002
- Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dotsNature Biotechnology, 2002
- In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid MicellesScience, 2002
- Nanocrystal targeting in vivoProceedings of the National Academy of Sciences, 2002
- Sorting Fluorescent Nanocrystals with DNAJournal of the American Chemical Society, 2002
- Conjugation of Luminescent Quantum Dots with Antibodies Using an Engineered Adaptor Protein To Provide New Reagents for FluoroimmunoassaysAnalytical Chemistry, 2002
- Colloidal Semiconductor Quantum Dot Conjugates in BiosensingPublished by Elsevier ,2002