Direct Isolation and Expansion of Human CD34+Hematopoietic Stem Cells
- 1 January 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Hematotherapy
- Vol. 2 (3) , 339-342
- https://doi.org/10.1089/scd.1.1993.2.339
Abstract
The isolation and expansion of CD34+ cells has numerous applications as supportive therapy for cytopenias that occur postchemotherapy. In this report, we describe a simple system to directly isolate and culture human CD34+ cells. Using this system, CD34+ cells isolated from bone marrow were cultured in the presence of GM-CSF, G-CSF, erythropoietin, stem cell factor, IL-1, IL-3, and IL-6. Cell expansions up to 100-fold were noted during the first 2 weeks of culture with high maintenance of CD34+ cells during the first week of culture. Maximal expansions of progenitors were observed at one week of culture, with, on average, 20-fold increases in CFU-GM number. The results support the feasibility of CD34+ cell expansion for clinical application.Keywords
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