NEAR-DIPLOID CLONES OF MOUSE CELLS - A SUITABLE TOOL FOR THE GENETIC-ANALYSIS OF MALIGNANCY

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (3) , 50-55
Abstract
Several near-diploid clones were obtained from the established mouse cell line, CAK-7. These clones proved to be highly oncogenic in syngeneic irradiated mice (TD50 [transplantation dose] = 1 .times. 102-2 .times. 103). They differed in plating efficiency in semi-solid medium (from 5 .times. 10-3 to 22 .times. 10-5). There was no correlation between tumorigenicity of the cells and their plating efficiency in methylcellulose. The differences between clones able to form anchorage-independent clonies would permit use for the genetic analysis of this kind of malignancy.